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KMID : 1103620150250030085
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Korean Journal of Aerospace and Environmental Medicine
2015 Volume.25 No. 3 p.85 ~ p.90
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Controlled Gene Expression System under Hypoxia Conditions
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Yun Yeo-Min
Ha Yoon
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Abstract
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Purpose: To understand the phenomenon of gene expression under hypoxia conditions, hypoxia-inducible vector system was constructed. Hypoxia-inducible enhancer and 3’ untranslated region was responsible to overexpress so that regulates gene expression under hypoxia conditions.
Methods: We used several kinds of hypoxia-inducible factors to regulate gene expression under hypoxia conditions. Erythropoietin (Epo) enhancer and 3’Epo-untranslate region (Epo-3’-UTR) was used respectively upstream or downstream of SV promoter. Mouse neural stem cells (mNSCs) transfected each vector was cultured under normoxia or hypoxia conditions.
Results: Luciferase assay and ELISA revealed that gene expression level under hypoxia conditions was higher than normoxia conditions. Plasmid containing both Epo enhancer and Epo-3’-UTR increased the best compared to normoxia conditions. However, Epo enhancer showed the highest gene expression under hypoxia conditions and relative to SV promoter.
Conclusions: As a result of gene expression level under hypoxia conditions compared to normoxia conditions, all of those 3 kinds vector have an effect on overexpression. On the basis of that, we can regulate gene expression under hypoxia conditions so that it can be applied to improve gene or cell therapy.
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KEYWORD
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Hypoxia, Gene expression regulation, Hypoxia-inducible, Enhancer
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